Epidermal-neural Crest Stem Cells Potential as a Therapy Candidate for Canine Spinal Cord Injury PDF
By:Barbara Gericota McMahill
Published on 2014 by
In man and animals spinal cord injury (SCI) is a serious neurological problem with severe and permanent debilitating neurological deficits, for which there currently is no cure. However, exciting progress in stem cell biology has provided the potential for revolutionary cell therapies. The use of a SCI model in a large animal such as the dog offers the major advantage of being a realistic model for human SCI. The embryonic neural crest has the ability to generate an astonishing variety of cell types and tissues in the adult vertebrate organism. Therefore neural crest stem cells (NCSC) are a very attractive tool in regenerative medicine. Epidermal neural crest stem cells (EPI-NCSC) are remnants of the embryonic neural crest in the bulge of the hair follicle. Furthermore, the close ontological relationship with the spinal cord makes EPI-NCSC an attractive candidate for cell-based therapies in models of SCI. The objective of this thesis was to perform the baseline research work to validate canine EPI-NCSC (cEPI-NCSC) as a candidate for SCI therapy in dogs. Chapter one reviews the literature on stem cell therapy for canine SCI and emphasizes the importance of dogs as a translational model for human disease. Experimental approaches using stem cells in canine SCI are compared, and clinical trials being conducted in veterinary medicine for canine SCI are summarized. Chapter two focuses on the isolation, expansion and characterization of cEPI-NCSC from adult dog hair follicles. The neural crest origin of cEPI-NCSC was verified and multipotency and self-renewal capability was demonstrated by in vitro clonal analysis. cEPI-NCSC can generate all major neural crest derivatives. Novel research tools suitable for qPCR with dog tissue were developed.Chapter three describes the fate, survival, and safety of transplanted cEPI-NCSC in a small number of healthy dogs. The spinal surgery technique for safe transplantation of cEPI-NCSC was developed. There were no longterm adverse effects associated with cell transplantation. cEPI-NCSC were successfully traced by magnetic resonance imaging (MRI) for 3 weeks. Grafted cEPI-NCSC integrated into the spinal cord, survived, did not migrate or form tumors. cEPI-NCSC may secreete neurotrophins, other growth factors, including factors that promote angiogenesis and factors that promote tissue remodeling.
This Book was ranked at 12 by Google Books for keyword Veterinary Medicine Small Animal Medicine novel.
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Book which was published by since 2014 have ISBNs, ISBN 13 Code is 9781321363333 and ISBN 10 Code is 1321363338
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